COMPARATIVE ANALYSIS OF MICROBIAL LOAD OF THE ENUGU MAIN WATER PRODUCTION AND WATER AVAILABLE TO IMT CAMPUS II HOSTEL
Samples of water from the different hostels in campus II and other source of water production namely Ajali-owa water, from bore hole 9th mile and spring water from GRA water board were water collecterd and analysed using total plate count and multiple tube fermentaton techniques coliform. The results shows no coliforms in production water from Ajali-owa and in GRA spring water reservaiour and no coliform in 9th mile bore hole water, 14 organism and 149 cfu/ml plate counts were obtained from tap water in IMT campus II hostel 7 coliform and Tocful / ml plate count from well water and 28 coliform and 280 cfu/ml plate count from spillage waste water. These results obtained show that tap water consumed in IMT campus II did not meet with NAFDAC and WHO standards. Preventive measures such as proper treatment of these water should be encourage and all pipes in campus II should be checked for leakages
TABLE OF CONTENT
Certification
Dedication
Acknowledgement
Abstract
Table of content
1.0 CHAPTER ONE
Introduction
1.1 Aims / objectives of the study
1.2 Statement of problems
1.3 Hypothesis
1.4 Scope of limitation
CHAPTER TWO
Literature review
2.1 The major group of biological Pollutions of water
2.2 Source of water supply
2.3 Source of water pollution
2.4 Treatment of water sample
2.5 International standard for drinking water
2.6 Diseases from water
CHAPTER THREE
3.0 Materials & methods
3.0 Materials
3.1 Media preparation
3.2 Collection of samples
3.3 Collection of samples
3.4 Methods of water analysis
3.5 Determination of PH
3.5.1 Determination of bacteria of the coliform
3.5.2 Culture techniques
3.5.3 Plate count technique
3.5.4 Presumption coliform (multiple tube fermebtation
3.5.4 Confirmatory test
3.5.5 Completed test
3.5.6 Test for indole
3.5.7 Methyl red test
3.5.8 Sugar test
3.5.9 Oxidase test
3.5.10 Catakase test
3.5.11 The slide cougulase test
3.5.12 Gram stain techniques
3.5.13 Detection of faecal stredtococci
3.5.14 Detection of escherichi coli test
3.5.15 Organism suspected after gram staining
CHAPTER FOUR
RESULTS
CHAPTER FIVE
Discussion
CHAPTER SIX
6.1 Conclusion
6.2 Recommendation
References
INTRODUCTION
Water is a liquid mineral. It consist of two atoms of hydrogen combined with one atom of oxygen. Water is a tasteless, odourless and colourless mobile liquid except in large volume where it appears blue. It has a melting point of OoC (320f) and a boiling point of 1000C (2120f). water is a basic necessity of life, in the absence higher animals survive only but a few hours or days. It has several uses such as washing cooking, food processing ,swimming, and among others. Out of these uses, drinking of water seems to be the most sensitive as it could have a direct deleterious impact on health of human beings.
Therefore, drinking water should be potable free diseases, or toxic substance (Beren 1991) .
Water is portable when it is colourless. Odourless, or tasteless and also free from poisonous, corroding, staining substances as well as disease casuiong oirganisms (Cruich 1972). The problem of providing safe and adequate water is as old as life on earth. Water can be made unifit or unsafe for drinking it contamination occurs either by nature or activities of human beings.
Perhaps the greatest danger associated with drinking water is contamination by human excrement (Forest 1979). In most cases, the pollution/contamination is hardly severe and is not particularly detrimental to health.
Thus a few substances and microbes that are health hazard do occur in water, and can cause illness or even death. From microbiological point
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