ABSTRACT
This research is aimed at isolating, partially purifying and determination of arginase from the kidney of a local goat. Methods used in the isolation and partial purification of the enzyme include; Ammonium sulphate precipitation, dialysis.etc. Result from this work showed that arginase isolated from a local goat has a specific activity of 0.358 with a yield of 12.12% incubation of the enzyme with different metal ions shows that the enzyme is inhibited by Nacl and Bacl2.
TABLE OF CONTENTS
CHAPTER ONE
1.0 Introduction and Literature review
1.1 Introduction
1.2 Literature review
1.2.1 History of arginase
1.3 Occurrence and distribution of arginase
1.3.1 Bacteria
1.3.2 Fungi
1.3.3 Lichens
1.3.4 Yeast
1.3.5 Insects
1.4 The substrate: Arginase
1.5 Scientific classification of local goat
1.6 Aim and Objectives
CHAPTER TWO
2.0 Materials and methods
2.1 Materials
2.1.1 Apparatus used
2.2 Methods
2.2.1 Assay
2.3.2 Protein determination
2.3.3 Purification procedure
CHAPTER THREE
3.0 Results
3.1 Purification and Homogeneity
3.2 Effect of metal ion on arginase from kidney of a local goat
CHAPTER FOUR
5.0 Discussion, Conclusion and Recommendation
5.1 Discussion
5.2 Conclusion
5.3 Recommendations
References.
Terms of Use: This is an academic paper. Students should NOT copy our materials word to word, as we DO NOT encourage Plagiarism. Only use as a guide in developing your original research work. Thanks.
Disclaimer: All undertaking works, records, and reports posted on this website, eprojectguide.com are the property/copyright of their individual proprietors. They are for research reference/direction purposes and the works are publicly supported. Do not present another person’s work as your own to maintain a strategic distance from counterfeiting its results. Use it as a guide and not duplicate the work in exactly the same words (verbatim). eprojectguide.com is a vault of exploration works simply like academia.edu, researchgate.net, scribd.com, docsity.com, course hero, and numerous different stages where clients transfer works. The paid membership on eprojectguide.com is a method by which the site is kept up to help Open Education. In the event that you see your work posted here, and you need it to be eliminated/credited, it would be ideal if you call us on +2348064699975 or send us a mail along with the web address linked to the work, to eprojectguide@gmail.com. We will answer to and honor each solicitation. Kindly note notification it might take up to 24 – 48 hours to handle your solicitation.