bacteria
Micro-organisms are ubiquitous and are found in almost every area around human bodies. Some are specifically found in certain regions of the body as a normal flora where they live as commensals with man. A this association is important in protecting the body against other infectious diseases.
each area of the body surface acquires a characteristic flora of organisms well adapted to growth at that particular environment these residents (normal flora) tend to suppress the intruders either by competition for space and food supply or by production of metabolites that are antagonistic to the survival of the intruder.
these residents could be dislodged from their environment when sneezing, coughing, belching, yawning or could be destroyed by regular use of antiseptic soaps or creams on the body surfaces.
handkerchiefs often used in males for wiping face, closing of the mouth and nose when expressing these reflex activities, therefore constitute an abode for bacteria furthermore, bacteria found in handkerchiefs could differ from one individual to another as the bacteria found could be a reflective of the environment and pathological conditions of the individual using the handkerchief for instance, individual with upper respiratory tract infection are likely to dislodge strains of pathogenic microbes along sides with the normal flora in these regions. enumeration of bacteria on used handkerchief in males can be done using microscopic cell count and viable cell accounting microscopic counts can be done on either samples dried on slides or samples in liquid. Viable cell counting is the one that is able to divide and form offspring. viable cell counting is also called plate count and there are at least two ways of performing plate count: the spread plate and pour plate method. In spread plate method, a volume of appropriately diluted culture is spread over the surface of an agar plate using a sterile glass spreader. The plate is then incubated until colonies appear, and the number of colonies formed are counted.
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